Harmful in conact with skin (buffers)
Electrical hazards (electrophoresis tank, microwave, UV transilluminator)
UV hazards (UV transilluminator)
Burning hazard (boiling agarose)
Ergonomic hazard (pipetting)
This requires UV safety training, and working with electricity.
Ensure chemical spill kit and first aid kit is within reach and that eye wash stations and safety showers are easily accessible.
Do not seal the flask when microwaving agarose! Steam needs to escape so pressure is not built up from boiling agarose.
Take care with SYBRSafe (or any gel stain alternative, such as Ethidium Bromide). Check local guidelines for disposal of these chemicals.
Do not pour liquid agarose down the sink.
Spill response: apply full PPE, mop up with absorbant material (e.g., paper towel), place in a sealed container and discard in a biohazard bin for incineration.