May 20, 2024
10x v3.1 HT Genomics Sample Processing Protocol
- Allen Institute1
- 1Allen Institute
Protocol Citation: Allen Institute 2024. 10x v3.1 HT Genomics Sample Processing Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov19o6klr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 20, 2024
Last Modified: May 20, 2024
Protocol Integer ID: 100145
Keywords: 10x, 10xV3.1, 10xV3.1_HT, GEX
Abstract
Protocol is used for rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of cells. Doubling the capacity of the original 10xv3.1 protocol, allowing for a capture of ~20,0000 cells instead of ~10,000 cells per port.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number
1U01MH114812-01
1U19MH114830-01. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
