Mar 26, 2020

Public workspace10X TBS Buffer

DOI
dx.doi.org/10.17504/protocols.io.bd85i9y6
  • 1Purdue University
  • Kelleher Research Group
    Tech. support email: kelleher-ofc@northwestern.edu
Bryon Drown
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DOI: dx.doi.org/10.17504/protocols.io.bd85i9y6
Protocol Citation: Bryon Drown 2020. 10X TBS Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.bd85i9y6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 25, 2020
Last Modified: March 26, 2020
Protocol Integer ID: 34813
Abstract
Tris buffered saline (TBS) is a commonly used buffer with a wide range of applications in biochemistry. It is often more convenient to prepare a 10X solution so that additional components can be added when preparing the buffer on demand.
Materials
MATERIALS
ReagentSodium ChlorideFisher ScientificCatalog #S271
ReagentTris BaseFisher ScientificCatalog #BP152
ReagentTris HydrochlorideFisher ScientificCatalog #BP153
ReagentHydrochloric AcidFisher ScientificCatalog #A144S
Safety warnings
Concentrated hydrochloric acid is corrosive by all exposure routes. Always work with concentrated HCl in a fume hood while wearing appropriate PPE.
Weigh out Tris base Amount3.13 g

Weigh out Tris HCl Amount27.45 g

Weigh out sodium chloride Amount88 g

Dissolve in MilliQ water Amount900 mL

Measure and adjust to Ph7.5 with concentrated HCl and/or NaOH

Add MilliQ water to a final volume of 1 L