Aug 11, 2023
10x Protocols: Visium v2 CytAssist FFPE Deparaffinization, H&E staining, and Imaging-- University of Minnesota TMCs (CG000520 Rev B)
- IOx Genomics1,
- Laura J Niedernhofer2,
- David A Bernlohr2
- 110x Genomics, info@10xgenomics.com;
- 2University of Minnesota, Minneapolis, MN US
Protocol Citation: IOx Genomics, Laura J Niedernhofer, David A Bernlohr 2023. 10x Protocols: Visium v2 CytAssist FFPE Deparaffinization, H&E staining, and Imaging-- University of Minnesota TMCs (CG000520 Rev B). protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpjpyjgzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 05, 2023
Last Modified: August 11, 2023
Protocol Integer ID: 81461
Keywords: UMN, 10x, Visium, CytAssist, FFPE, Spatial, UMinnesota, University of Minnesota, Niedernhofer, Bernlohr
Funders Acknowledgement:
NIH
Grant ID: 1U54AG07041-01
NIH
Grant ID: 1U54AG079754-01
Abstract
Protocols from 10x Genomics for Visium Spatial Gene Expression v2 chemistry on FPPE samples with the CytAssist component.
Protocol ID# (CG) and Revision used:
10x protocol CG000520, Revision B -- Deparaffinization, H&E staining, and Imaging prior to Library construction with CytAssist.
Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment.
10x protocol CG000520, Revision B (Deparaffinization, H&E staining and Imaging prior to Library Construction and CytAssist)
10x CytAssist Visium Deparaffin HE, Rev B (Protocol CG000520).pdf Additional Protocols/Resources