Aug 11, 2023
10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation -- University of Minnesota TMCs (CG000478 Rev B)
- IOx Genomics1,
- Laura J Niedernhofer2,
- David A Bernlohr2
- 110x Genomics, info@10xgenomics.com;
- 2University of Minnesota, Minneapolis, MN USA
Protocol Citation: IOx Genomics, Laura J Niedernhofer, David A Bernlohr 2023. 10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation -- University of Minnesota TMCs (CG000478 Rev B). protocols.io https://dx.doi.org/10.17504/protocols.io.81wgby3x3vpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 03, 2023
Last Modified: August 11, 2023
Protocol Integer ID: 81381
Keywords: 10x, scRNAseq, snRNAseq, sc/snRNAseq, Fixed, UMN
Funders Acknowledgement:
NIH
Grant ID: 1U54AG07041-01
NIH
Grant ID: 1U54AG079754-01
Abstract
DOIs for dissociation protocols and 10x Genomics fixation for Chromium Single Cell Expression flex protocols.
Please see DOIs for dissociation protocols linked here.
Protocol ID# (CG) and Revision letter provided here:
10x CG000478, Revision B -- Fixation of single cells and nuclei prior to applying 10x Genomics Chromium flex protocols.
Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
10x Protocol CG000478, Rev B (Fixation):
10x Cell+Nuclei Fixation Chromium Fixed RNA Profiling, Rev B (Protocol CG000478).pdf