Jun 26, 2023
10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex-- University of Minnesota TMCs (CG000527 Rev C)
- IOx Genomics1,
- Laura Niedernhofer2
- 110x Genomics, info@10xgenomics.com;
- 2University of Minnesota Medical School, Minneapolis, MN, USA
Protocol Citation: IOx Genomics, Laura Niedernhofer 2023. 10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex-- University of Minnesota TMCs (CG000527 Rev C). protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8jy4dg2w/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 11, 2023
Last Modified: June 26, 2023
Protocol Integer ID: 81752
Keywords: 10x, scRNAseq, snRNAseq, sc/snRNAseq, Fixed, UMN, University of Minnesota, UMinnesota
Abstract
10x Genomics Chromium Single Cell Expression flex protocol for library construction.
Protocol ID# (CG) and Revision letter provided here:
10x protocol CG000527, Revision C -- Library construction of 10x fixed single cell or nuclei
Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
Complete single cell or nuclei isolation and 10x fixation prior to starting this protocol
10x Protocol CG000527, Revision C (Library Construction):
10x Chromium Fixed RNA Profiling, Multiplexed Samples, Rev C (Protocol CG000527).pdf