May 18, 2023

Public workspace10x Genomics Multiome ATAC+GEX 

DOI
dx.doi.org/10.17504/protocols.io.n2bvj8kqpgk5/v1
  • 1Department of Medicine, Washington University in St. Louis
Andrew Houston
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DOI: dx.doi.org/10.17504/protocols.io.n2bvj8kqpgk5/v1
Protocol Citation: Andrew Houston 2023. 10x Genomics Multiome ATAC+GEX . protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj8kqpgk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 18, 2023
Last Modified: May 18, 2023
Protocol Integer ID: 82115
Abstract
Link to 10x Genomics Multiome protocol to assess chromatin accessibility and gene expression from a dissociated sample
Protocol can be found here:
https://cdn.10xgenomics.com/image/upload/v1666737555/support-documents/CG000338_ChromiumNextGEM_Multiome_ATAC_GEX_User_Guide_RevF.pdf

Transposition through cDNA pgs. 28-57
ATAC library construction pgs. 49-52
GEX library construction pgs. 59-67