Nov 07, 2014
Version 1
10X RT Buffer V.1
- New England Biolabs (NEB)Tech. support phone: +1(800)632-7799 email: info@neb.com
External link: https://www.neb.com/protocols/1/01/01/first-strand-synthesis-protocol-with-reverse-transcriptase
Protocol Citation: New England Biolabs: 10X RT Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.cjwupd
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: November 07, 2014
Last Modified: March 28, 2018
Protocol Integer ID: 342
500 mM Tris-HCl (pH 8.3 @ 25°C)
750 mM KCl
30 mM MgCl2
100 mM DTT