Jun 07, 2024
100ml EB buffer (PH=8)
This protocol is a draft, published without a DOI.
- Menglin WANG1
- 1UC Berkeley
Protocol Citation: Menglin WANG 2024. 100ml EB buffer (PH=8). protocols.io https://protocols.io/view/100ml-eb-buffer-ph-8-de853hy6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 06, 2024
Last Modified: June 07, 2024
Protocol Integer ID: 101373
Abstract
This is the recipe for how to make 100ml EB buffer (PH=8)
Protocol materials
UltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015
In 2 steps
Tris BaseFisher ScientificCatalog #BP152-1
Step 2
100ml EB buffer (10mM Tris-HCl) pH=8
100ml EB buffer (10mM Tris-HCl) pH=8
Prepare 80 mL UltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015 in a suitable container.
Add 0.12114 g of Tris BaseFisher ScientificCatalog #BP152-1 to the solution.
Adjust solution to desired (pH 8.0) using HCl (typically pH ≈ 7.0).
Add UltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015 until the volume reaches to 100ml.