Jun 07, 2024
100ml 5M Sodium Chloride (NaCl)
This protocol is a draft, published without a DOI.
- Menglin WANG1
- 1UC Berkeley
Protocol Citation: Menglin WANG 2024. 100ml 5M Sodium Chloride (NaCl). protocols.io https://protocols.io/view/100ml-5m-sodium-chloride-nacl-dfa43igw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 07, 2024
Last Modified: June 07, 2024
Protocol Integer ID: 101436
Abstract
This is a recipe for how to make 100ml 5M Sodium Chloride (NaCl).
Protocol materials
Sodium ChlorideFisher ScientificCatalog #S271
In 2 steps
UltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015
In 2 steps
5M Sodium Chloride (NaCl,100ml)
5M Sodium Chloride (NaCl,100ml)
20m
Weigh out an amount of 29.22 g of Sodium ChlorideFisher ScientificCatalog #S271 in an appropriate-size beaker or conical flask (1L volume). Add 80 mL of UltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015 .
Precaution: Do not add 100 ml of deionized/Milli-Q water. In most cases, solution volume increases when a large amount of solute is dissolved in a solvent.
Dissolve Sodium ChlorideFisher ScientificCatalog #S271 using a magnetic stirrer or manual shaking with a glass stirring rod.
Tip: You can heat the solution to 60 °C to accelerate the dissolution process.
Transfer the solution to a measuring cylinder or a volumetric flask and adjust the volume to 100 mL with UltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015 . Remix it.
Transfer the solution to an autoclavable bottle and sterilize it by autoclaving 00:20:00 at 15 lb/sq.in. (psi) from 121-124 °C on liquid cycle.
20m
Storage: Store the solution at Room temperature .
Tip: NaCl may precipitate with time. Dissolve it by shaking the bottle vigorously.