Jul 13, 2023

Public workspace0.3M Sodium Cacodylate Buffer pH 7.4 Stock Solution Recipe

DOI
dx.doi.org/10.17504/protocols.io.q26g74x79gwz/v1
  • Mark Ellisman1,2,
  • Marianne Martone1,
  • Thomas Deerinck1,2
  • 1National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla CA;
  • 2Department of Neurosciences, University of California, San Diego, La Jolla CA
National Center for Microscopy and Imaging Research
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DOI: dx.doi.org/10.17504/protocols.io.q26g74x79gwz/v1
Protocol Citation: Mark Ellisman, Marianne Martone, Thomas Deerinck 2023. 0.3M Sodium Cacodylate Buffer pH 7.4 Stock Solution Recipe. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g74x79gwz/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 26, 2022
Last Modified: July 13, 2023
Protocol Integer ID: 61479
Keywords: cacodylate , buffer, stock, solution
Abstract
Standard preparation protocol for stock 0.3M sodium cacodylate buffer.
Guidelines
Cacodylate acid, sodium salt, trihydrate (MW = 214.03)
Vendor: Ted Pella
Product number: 18851

Calculation:

M (moles/liter) = g/ MW (g/mol) X V (liter)

0.3M = g/214.03 g/moles X 1.0L

g = M x V x MW

g = 0.3 moles/liter X 214.03 g/moles X 1.0 liter

g = 64.209 grams for 1.0 liter

For 3L = 3 x 64.209 = 192.627 grams
Materials
- Sodium Cacodylate trihydrate salt [TED PELLA, INC. (Catalog no. 18851)]
- Double distlled H2O (ddH2O)
- Appropriately sized container
- Concentrated hydrochloric acid


Safety warnings
Always wear appropriate PPE.
Cacodylate is always toxic.
Add 900 ml of DDH2O to 64.209 grams of sodium cacodylate in a large 1.0L or greater container, such as a capped glass bottle. Shake and wait until powder is fully dissolved.

Maximum HCL concentration is 12.18mole/liter (36-38%). Start with 1.3mL of 12.18 M HCL per 900 ml of sodium cacodylate dissolved solution. Make sure solution is mixed thoroughly.

Check pH. Keep adding 50-100μl of HCL, mix, and check pH again until pH 7.4 has been reached. (Approximately 1.4 ml of conc. HCL total).
Top off to 1000ml with DDH2O.
Do a final pH check and keep stock solution refrigerated at 4C.