Reagent/consumable | Supplier | Catalogue number | |
Reagents | |||
Water | Sigma Aldrich/Merck | 1153332500 | |
5 M NaCl | Sigma Aldrich/Merck | S5150-1L | |
1 M Tris-HCl, pH 8.0 | AppliChem | A4577,1000 | |
0.5 M EDTA, ph 8.0 | AppliChem | A4892,1000 | |
Tween-20 | Thermo Fisher Scientific | 11417160 | |
20% SDS | Thermo Fisher Scientific | AM9820 | |
Consumables | |||
Square media bottle 500 ml | VWR | 391-0630 | |
50 ml serological pipet | Corning BV | 357550 | |
10 ml serological pipet | Corning BV | 357551 | |
5 ml serological pipet | Corning BV | 357543 |
Reagent | Volume | Final concentration in reaction | |
Water | 471.25 ml | ||
5 M NaCl | 10 ml | 100 mM | |
1 M Tris-HCl, pH 8.0 | 5 ml | 10 mM | |
0.5 M EDTA, ph 8.0 | 1 ml | 1 mM | |
100% Tween-20 | 0.25 ml | 0.05% | |
20% SDS solution | 12.5 ml | 0.50% | |
sum | 500 ml |