Apr 10, 2020

Public workspace0.1% Cresyl Violet Stain (pH 4.3)

DOI
dx.doi.org/10.17504/protocols.io.wcjfaun
  • 1Thomas Jefferson University
  • SPARC
    Tech. support email: info@neuinfo.org
Shaina Robbins
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DOI: dx.doi.org/10.17504/protocols.io.wcjfaun
Protocol Citation: Shaina Robbins, Sean Nieves 2020. 0.1% Cresyl Violet Stain (pH 4.3). protocols.io https://dx.doi.org/10.17504/protocols.io.wcjfaun
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 11, 2018
Last Modified: April 10, 2020
Protocol Integer ID: 18539
Materials
MATERIALS
ReagentEMD Millipore™ Ultrapure Water for Molecular BiologyEmd MilliporeCatalog #09-739-011
ReagentCresyl Violet acetate pure high purity biological stainCatalog #AC229630050
ReagentAcetic Acid Glacial Catalog #A38-212
STEP MATERIALS
ReagentCresyl Violet acetate pure high purity biological stainCatalog #AC229630050
ReagentAcetic Acid Glacial Catalog #A38-212

Protocol materials
ReagentEMD Millipore™ Ultrapure Water for Molecular BiologyMerck Millipore (EMD Millipore)Catalog #09-739-011
Materials
ReagentCresyl Violet acetate pure high purity biological stainCatalog #AC229630050
In Materials, Materials, Step 2
ReagentAcetic Acid Glacial Catalog #A38-212
In Materials, Materials, Step 4
Safety warnings
Signal Word: Warning
Hazard Statements: Causes skin irritation; Causes serious eye irritation; May cause respiratory irritation
  • Prevention: Wash face, hands and any exposed skin thoroughly after handling Wear protective gloves/protective clothing/eye protection/face protection Avoid breathing dust/fume/gas/mist/vapors/spray Use only outdoors or in a well-ventilated area
  • Inhalation: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing Call a POISON CENTER or doctor/physician if you feel unwell
  • Skin:IF ON SKIN: Wash with plenty of soap and water If skin irritation occurs: Get medical advice/attention Take off contaminated clothing and wash before reuse
  • Eyes: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing If eye irritation persists: Get medical advice/attention
  • Storage: Store in a well-ventilated place. Keep container tightly closed Store locked up
  • Disposal: Dispose of contents/container to an approved waste disposal plant
Solution Prep
Solution Prep
Measure Amount500 mL of ddH2O or molecular grade H2O and pour into a glass beaker

Aliquot Amount0.5 g of cresyl violet acetate into the glass beaker from the above step
ReagentCresyl Violet acetate pure high purity biological stainCatalog #AC229630050

Safety information
Causes skin irritation
May cause respiratory irritation
Causes serious eye irritation


Put a magnetic stir bar into the glass beaker (containing H2O and cresyl violet acetate) and place on a hot plate at Temperature100 °C and stir until cresyl violet is dissolved

Note
Allow solution to come to room temperature once cresyl violet is dissolved

pH
pH
Test the pH of the solution. Add glacial acetic acid dropwise until the desired (pH 4.3) is achieved

ReagentAcetic Acid Glacial Catalog #A38-212

Filter
Filter
Pour the contents of the 0.1% Cresyl Violet solution through a sterile membrane filter into a light sensitive jar and close lid