Mar 26, 2025
0.01M Phosphate buffered saline (1L)
This protocol is a draft, published without a DOI.
- 1University of Edinburgh
Protocol Citation: Thomas R Clarke 2025. 0.01M Phosphate buffered saline (1L). protocols.io https://protocols.io/view/0-01m-phosphate-buffered-saline-1l-cimcuc2w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: October 31, 2022
Last Modified: March 26, 2025
Protocol Integer ID: 72068
Abstract
Guide for making 0.01M PBS.
Used as the solution for slicing brains in the vibratome, and for transcardial perfusionsTHIS IS A DRAFT PROTOCOL - PLEASE EXERCISE CAUTION
Measure ~900mL distilled water
2m
Add 5 phosphate buffered saline tablets (Sigma) and stir with magnetic flea
5m
Fill up to 1000ml with distilled water
2m
Measure pH and adjust to 7.4
5m